Journal: Parkinson's Disease

Article Title: The Neuroprotective Effect of Isotetrandrine on Parkinson's Disease via Anti-Inflammation and Antiapoptosis In Vitro and In Vivo

doi: 10.1155/2023/8444153

Figure Lengend Snippet: Effect of isotetrandrine (ITD) on LPS-induced iL-6, iNOS, and COX-2 mRNA and protein expression in BV2 cell. BV2 microglia cells were cotreated with LPS and different concentrations of isotetrandrine (ITD) (200, 100, and 10 μ M) for 24 hr and harvested for western blots and qPCR. (a) Western blots for iNOS, COX-2, and β -actin protein expression in each group. (b) Quantification results of relative density of iNOS and COX-2 expression in each group. The relative density of the LPS-induced group was taken to be 100%. β -actin was used as an internal control. (c–f) Quantitative PCR of interleukin-6 ( Il-6 ), inducible nitric oxide synthase ( Inos ), and cyclooxygenase-2 ( Cox-2 ) and cd11b mRNA expression in each group. Gapdh was used as an internal control. Data are presented as mean ± SEM ( n = 3). ∗ Significantly different from the control group; p < 0.05; # significantly different from the LPS group.

Article Snippet: The cells were maintained with BV2 cells complete medium (Elabscience, USA, TX, No. CM-0493) in a 5% CO 2 37°C incubator.

Techniques: Expressing, Western Blot, Control, Real-time Polymerase Chain Reaction